mouse anti s100 β antibody  (Proteintech)

Journal: iScience

Article Title: Benzyl isothiocyanate suppresses development of thyroid carcinoma by regulating both autophagy and apoptosis pathway

doi: 10.1016/j.isci.2024.110796

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-S100 , Santa Cruz Biotechnology , sc-393919 lot# C1120; RRID: AB_2910203.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Software

Journal: bioRxiv

Article Title: CRISPRi-based screens in iAssembloids to elucidate neuron-glia interactions

doi: 10.1101/2023.04.26.538498

Figure Lengend Snippet: (A) hiPSCs expressing CRISPRi machinery and inducible NGN2 are pre-differentiated and seeded with hiPSC-derived astrocytes at a 3:1 neurons:astrocyte ratio. After 1 week, hiPSC-derived microglia are seeded at one-third of the number of astrocytes. (B) Brightfield images of iAssembloids 30 min, 1 day, 1 week and 2 weeks post seeding. Scale bar = 800 µm. (C) Neurons (gray), astrocytes (green) and microglia (red) expressing different fluorescent proteins were seeded to form iAssembloids. Left image: maximum-intensity projection, other images: individual images from the horizontal sample images (z-stack) generated from confocal microscopy. Arrows denote neurons co-localized with microglia (closed arrowheads) as well as neuronal extensions across the culture (open arrowheads). Scale bar = 50 µm. Images were taken 14 days post seeding into AggreWell 800 plates. (D) Maximum intensity projections of iAssembloids stained with antibodies against neuronal markers NEUN and TUJ1. Scale bar = 50 µm. Images were taken 14 days post seeding into AggreWell™ 800 plates. (E) Maximum intensity projections of iAssembloids stained with antibodies against the microglial marker IBA1 and the astrocyte marker S100β. Scale bars = 50 µm. Images were taken 14 days post seeding into AggreWell™ 800 plates. Arrows denote microglia projections.

Article Snippet: Primary antibodies used for this study were as follows: rabbit anti-Iba1 (Wako, Cat. No. 019-19741), mouse anti-NeuN, clone A60 (MilliporeSigma, Cat. No. MAB377), mouse anti-S100 (β-Subunit) (MilliporeSigma, Cat. No. S2532), chicken anti-Tuj1 (Aves Labs, Cat. No. TUJ-0020), rabbit anti-NRF2 (Abcam, Cat. No. ab62352).

Techniques: Expressing, Derivative Assay, Generated, Confocal Microscopy, Staining, Marker